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更新于2024年12月18日
材料与资源
质粒
发表的质粒可在 Addgene，WeKwikGene和 MolecularCloud 获得。 对于未发表的新版乙酰胆碱、多巴胺、五羟色胺、去甲肾上腺素、内源大麻素等探针，请联系 yulonglilab2018(a)gmail.com 以获取相关信息。
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GRAB sensors列表
| 类别 | 配体 | 版本名 | 最大反应与 亲和力 |
受体骨架 | 光谱信息 | 论文链接 | 备注 |
| Green 5-HT sensor | 5-HT | 5-HT1.0 | 280%, 22 nM | human HTR2C | / | https://doi.org/10.1038/s41593-021-00823-7 | / |
| 5-HTmut | / | human HTR2C | / | / | |||
| 5-HT3.0 | 1300%, 150 nM | mouse HTR4 | Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm | https://doi.org/10.1038/s41592-024-02188-8 | Highly recommend | ||
| 5-HT3.0mut | / | mouse HTR4 | / | / | |||
| 5-HT2m | 1400%, 1.1 uM | mouse HTR4 | Peak: Ex 505 nm, Em 520 nm Isobestic point: 410 nm | / | |||
| 5-HT2h | 600%, 80 nM | mouse HTR4 | Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm | / | |||
| Red 5-HT sensor | r5-HTmut | / | mouse HTR4 | / | / | ||
| r5-HT1.0 | 400%, 790 nM | mouse HTR4 | Peak: Ex 560 nm, Em 595 nm | Highly recommend, previous internal name r5-HT2.0, nondetectable photoactivation | |||
| Green ACh sensor | ACh | gACh2.0 | 90%, 2 uM | human M3R | / | https://doi.org/10.1038/nbt.4184 | / |
| gACh3.0 (ACh4.3) | 280%, 2.2 uM | human M3R | Peak: Ex 492 nm, Em 510 nm | https://doi.org/10.1038/s41592-020-0953-2 | Highly recommend | ||
| gACh3.0-mut | / | human M3R | / | / | |||
| Green DA sensor | DA | DA1m | 90%, 130 nM | human D2R | / | https://doi.org/10.1016/j.cell.2018.06.042 | / |
| DA1m-mut | / | human D2R | / | / | |||
| DA1h | 90%, 10 nM | human D2R | / | / | |||
| DA1h-mut | human D2R | / | / | ||||
| DA2m | 340%, 90 nM | human D2R | Peak: Ex 500 nm, Em 520 nm Isobestic point: 435 nm | https://doi.org/10.1038/s41592-020-00981-9 | Spectrum source data | ||
| DA2h | 280%, 7 nM | human D2R | Peak: Ex 500 nm, Em 520 nm Isobestic point: 440 nm | Spectrum source data | |||
| gDA3m | 1300%, 86 nM | human D1R | Peak: Ex 495 nm, Em 520 nm Isobestic point: 420 nm | https://doi.org/10.1038/s41592-023-02100-w | Highly recommend | ||
| gDA3h | 1000%, 22 nM | bovine D1R | Peak: Ex 505 nm, Em 520 nm Isobestic point: 420 nm | Spectrum source data | |||
| Red DA sensor | rDA1m | 150%, 95 nM | human D2R | Peak: Ex 565 nm, Em 595 nm | https://doi.org/10.1038/s41592-020-00981-9 | / | |
| rDA1h | 100%, 4 nM | human D2R | / | / | |||
| rDA2m(D2) | 560%, 180 nM | red fire ant D2R | Peak: Ex 565 nm, Em 595 nm | https://doi.org/10.1038/s41592-023-02100-w | Highly recommend, Nondetectable photoactivation | ||
| rDA2h(D2) | 240%, 9 nM | red fire ant D2R | Peak: Ex 565 nm, Em 595 nm | Nondetectable photoactivation | |||
| rDA3m(D1) | 1300%, 130 nM | human D1R | Peak: Ex 560 nm, Em 595 nm | Highly recommend | |||
| rDA3h(D1) | 900%, 20 nM | human D1R | Peak: Ex 565 nm, Em 585 nm | / | |||
| Green NE sensor | NE | NE1m | 230%, 930 nM | human α2AR | / | https://doi.org/10.1016/j.neuron.2019.02.037 | / |
| NE1h | 130%, 83 nM | human α2AR | / | / | |||
| NEmut | / | human α2AR | / | Non-binding mutant control | |||
| NE2m | 381%, 380 nM | human α2AR | Peak: Ex 500 nm, Em 520 nm Isobestic point: 410 nm | https://doi.org/10.1016/j.neuron.2024.03.001 | Highly recommend | ||
| NE2h | 415%, 190 nM | human α2AR | Peak: Ex 500 nm, Em 520 nm Isobestic point: 410 nm | / | |||
| Green HA sensor | HA | HA1h | 370%, 17 nM | human H4R | Peak: Ex 505 nm, Em 520 nm Isobestic point: 400 nm | https://doi.org/10.1016/j.neuron.2023.02.024 | / |
| HA1m | 590%, 380 nM | human H4R | Peak: Ex 505 nm, Em 520 nm Isobestic point: 420 nm | Highly recommend | |||
| HA1mut | / | human H4R | / | / | |||
| Green OA sensor | OA | OA1.0 | 660%, 200nM | Drosophila Oct β2R | Peak: Ex 500 nm, Em 520 nm | https://doi.org/10.1093/nsr/nwae112 | / |
| Green Ado sensor | Ado | Ado1.0 | 200%, 60 nM | human A2AR | / | https://doi.org/10.1126/science.abb0556 | Highly recommend for physiology |
| Ado1.0m | 400%, 3600 nM | human A2AR | / | https://doi.org/10.1073/pnas.2212387120 | Highly recommend for pathology | ||
| Green ATP sensor | ATP | ATP1.0 | 800%, 80 nM | human P2RY1 | Peak: Ex 500 nm, Em 520 nm Isobestic point: 435 nm | https://doi.org/https://doi.org/10.1016/j.neuron.2021.11.027 | / |
| ATP1.0L | 1000%, 32 uM | human P2RY1 | / | / | |||
| Green UDP sensor | UDP | UDP1.0 | 600%, 90 nM | chicken P2Y6 | Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm | https://doi.org/10.1016/j.neuron.2024.03.017 | / |
| Others | VIP | VIP1.0 | 360%, 19 nM | human VIPR2 | Peak: Ex 500 nm, Em 520 nm Isobestic point: 420 nm | https://doi.org/doi:10.1126/science.abq8173 | / |
| CCK | CCK1.0 (2.3) | 840%, 4.1 nM | human CCKBR | Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm | / | ||
| NPY | NPY1.0 | 490%, 43 nM | human NPY1R | Peak: Ex 500 nm, Em 520 nm Isobestic point: 425 nm | / | ||
| NPYmut | / | human NPY1R | / | / | |||
| CRF | CRF1.0 (3.0) | 1200%, 33 nM | human CRFR1 | Peak: Ex 500 nm, Em 520 nm Isobestic point: 415 nm | / | ||
| CRFmut | / | human CRFR1 | / | / | |||
| SST | SST1.0 | 430%, 70 nM | human SSTR5 | Peak: Ex 500 nm, Em 520 nm Isobestic point: 425 nm | / | ||
| NTS | NTS1.0 | 250%, 6.2 nM | human NTSR1 | Peak: Ex 500 nm, Em 520 nm Isobestic point: 425 nm | / | ||
| eCB | eCB2.0 | 300%, 0.2uM to AEA, 3uM to 2-AG | human CB1R | Peak: Ex 500 nm, Em 520 nm | https://doi.org/10.1038/s41587-021-01074-4 | / | |
| OT(Oxytocin) | OT1.0 | 410%, 3 nM | bovine OTR | Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm | https://doi.org/10.1038/s41587-022-01561-2 | / | |
| OTmut | / | bovine OTR | / | Non-binding mutant control |
果蝇
携带已发表的乙酰胆碱和多巴胺探针的转基因果蝇可以从Bloomington Drosophila Stock Center获得。
| Genotype | Cat# | Resource |
| cAMP sensor | ||
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-G-Flamp1.L}VK00005/TM2 | 604602 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-G-Flamp1.L}attP40 | 604603 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=UAS-G-Flamp1}attP40 / CyO; Sb[1] / TM6B, Tb[1] | 119070 | Kyoto stock center |
| w[*]; PBac{y[+mDint2] w[+mC]=UAS-G-Flamp1}VK00005 / TM3, Sb[1] | 119071 | Kyoto stock center |
| OA sensor | ||
| w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(OA1.0)}VK00005 | 604601 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(OA1.0)}attP40/CyO | 604600 | BDSC |
| ACh sensors | ||
| w[*]; P{w[+mC]=UAS-GRAB(ACh2.0)}2/In(2LR)Gla, wg[Gla-1] PPO1[Bc] | 80046 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(ACh3.0)}attP40 | 86549 | BDSC |
| w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(ACh3.0)}VK00005/TM6B, Tb[1] | 86550 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(ACh3.0)}attP40 | 86551 | BDSC |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(ACh3.0)}VK00005/TM2 | 86552 | BDSC |
| DA sensors | ||
| w[*]; P{w[+mC]=UAS-GRAB(DA1m)}2 | 80047 | BDSC |
| w[*]; P{w[+mC]=UAS-GRAB(DA1m)}3/TM2 | 80048 | BDSC |
| y[1] w[*]; P{y[+t7.7] w[+mC]=lexAop-GRAB(DA1m)}attP40/CyO | 80049 | BDSC |
| y[1] w[*]; PBac{y[+mDint2] w[+mC]=lexAop-GRAB(DA1m)}VK00005 | 80050 | BDSC |
| w[*]; P{w[+mC]=UAS-DA1m-mut}attP40/CyO | 80051 | BDSC |
| w[*]; P{w[+mC]=10XUAS-GRAB(DA2m)}attP40/CyO | 90878 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(DA2m)}attP40/CyO | 90879 | BDSC |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(DA2m)}VK00005/TM2 | 90880 | BDSC |
| 5-HT sensors | ||
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(5-HT1.0)}attP40 | 90874 | BDSC |
| w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(5-HT1.0)}VK00005 | 90875 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(5-HT1.0)}attP40/CyO | 90876 | BDSC |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(5-HT1.0)}VK00005 | 90877 | BDSC |
| PARIS | ||
| w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP40 | 82174 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP2 | 82175 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-pHluorin.CAAX}attP2 | 82176 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO | 82177 | BDSC |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}attP40 | 82178 | BDSC |
| w[*]; M{w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}ZH-86Fb | 82179 | BDSC |
病毒
多种已发表和未发表的探针病毒（包括最新的ACh, DA, NE, 5-HT, Ado, ATP, VIP, CCK, eCB，Ado，ATP探针)可以从布林凯斯，枢密公司，维真公司或泰儿图公司订购. 注意：订购未发表的探针病毒须提前联系李毓龙实验室 yulonglilab2018@gmail.com。
| Virus | Cat# |
| AAV-hSyn-GACh2.0 | PT-1309 |
| AAV-EF1α-DIO-GACh2.0 | PT-1310 |
| AAV-hSyn-DA1m | PT-1298 |
| AAV-EF1α-DIO-DA1m | PT-1299 |
| AAV-hSyn-DA1h | PT-1301 |
| AAV-EF1α-DIO-DA1h | PT-1302 |
| AAV-hSyn-DA mut | PT-1304 |
| AAV-EF1α-DIO-DA mut | PT-1305 |
| Virus | Cat# |
| pAAV-hSyn-GRAB_DA1m | 113049-AAV9 |
| pAAV-hSyn-GRAB_DA1h | 113050-AAV9 |
| pAAV-hSyn-GRAB_NE1m | 123308-AAV9 |
| pAAV-hSyn-GRAB_NE1h | 123309-AAV9 |
| pAAV-hSyn-GRAB_DA1h | 113050-AAVrg |
| pAAV-hSyn-GRAB_NE1m | 123308-AAVrg |
| pAAV-hSyn-GRAB_NE1h | 123309-AAVrg |
| pAAV-hSyn-GRAB_NEmut | 123310-AAVrg |
对于国外的研究者，请联系yulonglilab2018(atAT)gmail.com获取病毒订购的相关信息。