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Resources
Plasmids
Published plasmids are avalible at Addgene and MolecularCloud. For the latest unpublished sensors for ACh, DA, 5-HT, NE, eCB, Ado, ATP etc, please contact yulonglilab2018(a)gmail.com for information.
Drosophila
Transgenic flies carrying published ACh sensor and dopamine sensor are available at Bloomington Drosophila Stock Center.
| Genotype | Cat# |
| ACh sensors | |
| w[*]; P{w[+mC]=UAS-GRAB(ACh2.0)}2/In(2LR)Gla, wg[Gla-1] PPO1[Bc] | 80046 |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(ACh3.0)}attP40 | 86549 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(ACh3.0)}VK00005/TM6B, Tb[1] | 86550 |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(ACh3.0)}attP40 | 86551 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(ACh3.0)}VK00005/TM2 | 86552 |
| DA sensors | |
| w[*]; P{w[+mC]=UAS-GRAB(DA1m)}2 | 80047 |
| w[*]; P{w[+mC]=UAS-GRAB(DA1m)}3/TM2 | 80048 |
| y[1] w[*]; P{y[+t7.7] w[+mC]=lexAop-GRAB(DA1m)}attP40/CyO | 80049 |
| y[1] w[*]; PBac{y[+mDint2] w[+mC]=lexAop-GRAB(DA1m)}VK00005 | 80050 |
| w[*]; P{w[+mC]=UAS-DA1m-mut}attP40/CyO | 80051 |
| w[*]; P{w[+mC]=10XUAS-GRAB(DA2m)}attP40/CyO | 90878 |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(DA2m)}attP40/CyO | 90879 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(DA2m)}VK00005/TM2 | 90880 |
| 5-HT sensors | |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(5-HT1.0)}attP40 | 90874 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(5-HT1.0)}VK00005 | 90875 |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(5-HT1.0)}attP40/CyO | 90876 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(5-HT1.0)}VK00005 | 90877 |
| PARIS | |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP40 | 82174 |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP2 | 82175 |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-pHluorin.CAAX}attP2 | 82176 |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO | 82177 |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}attP40 | 82178 |
| w[*]; M{w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}ZH-86Fb | 82179 |
Viruses
AAV viruses carrying published and unpublished GRAB sensors (including the lastest ACh, DA, NE, 5-HT, Ado, ATP, VIP, CCK and eCB sensors) are available from Brain Case , BrainVTA (China)or WZ Biosciences (China) & WZ Biosciences (US). Note: please contact the Li Lab (yulonglilab2018@gmail.com) before ordering unpublished sensors.
| Virus | Cat# |
| AAV-hSyn-GACh2.0 | PT-1309 |
| AAV-EF1α-DIO-GACh2.0 | PT-1310 |
| AAV-hSyn-DA1m | PT-1298 |
| AAV-EF1α-DIO-DA1m | PT-1299 |
| AAV-hSyn-DA1h | PT-1301 |
| AAV-EF1α-DIO-DA1h | PT-1302 |
| AAV-hSyn-DA mut | PT-1304 |
| AAV-EF1α-DIO-DA mut | PT-1305 |
| Virus | Cat# |
| pAAV-hSyn-GRAB_DA1m | 113049-AAV9 |
| pAAV-hSyn-GRAB_DA1h | 113050-AAV9 |
| pAAV-hSyn-GRAB_NE1m | 123308-AAV9 |
| pAAV-hSyn-GRAB_NE1h | 123309-AAV9 |
| pAAV-hSyn-GRAB_DA1h | 113050-AAVrg |
| pAAV-hSyn-GRAB_NE1m | 123308-AAVrg |
| pAAV-hSyn-GRAB_NE1h | 123309-AAVrg |
| pAAV-hSyn-GRAB_NEmut | 123310-AAVrg |
For international users, please contact yulonglilab2018(a)gmail.com for information.
