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Updated on August 22, 2024
Resources
GRAB sensors list
| Category | Ligand | Version name | Peak response and EC50 | Paper link | Notes |
| Green 5-HT sensor | 5-HT | 5-HT3.0 | 1300%, 150 nM | https://doi.org/10.1038/s41592-024-02188-8 | Highly recommend |
| 5-HT2m | 1400%, 1.1 uM | ||||
| 5-HT2h | 600%, 80 nM | ||||
| Red 5-HT sensor | r5-HT1.0 | 400%, 790 nM | Highly recommend | ||
| Green ACh sensor | ACh | gACh3.0 (ACh4.3) | 280%, 2.2 uM | https://www.nature.com/articles/s41592-020-0953-2#article-info | |
| Green DA sensor | DA | gDA3m | 1300%, 100 nM | https://doi.org/10.1038/s41592-023-02100-w | Highly recommend |
| gDA3h | 1000%, 12 nM | ||||
| Red DA sensor | rDA2m | 600%, 140 nM | |||
| rDA2h | 300%, 6 nM | ||||
| rDA3m | 1300%, 40 nM | Highly recommend | |||
| rDA3h | 900%, 4 nM | ||||
| Green NE sensor | NE | NE2m | 400%, 400 nM | https://doi.org/10.1016/j.neuron.2024.03.001 | Highly recommend |
| NE2h | 400%, 100 nM | ||||
| Green ATP sensor | ATP | ATP1.0 | 800%, 80 nM | https://doi.org/https://doi.org/10.1016/j.neuron.2021.11.027 | |
| ATP1.0L | 1000%, 32 uM | ||||
| Green Ado sensor | Ado | Ado1.0 | 200%, 60 nM | https://science.sciencemag.org/content/369/6508/eabb0556 | Highly recommend for physiology |
| Ado1.0m | 400%, 3600 nM | https://doi.org/10.1073/pnas.2212387120 | Highly recommend for pathology |
||
| Green eCB sensor | eCB | eCB2.0 | 300%, 0.2uM to AEA, 3uM to 2-AG |
https://www.nature.com/articles/s41587-021-01074-4 | |
| Green OT sensor | OT | OT1.0 | 410%, 3 nM | https://doi.org/10.1038/s41587-022-01561-2 | |
| Green neuropeptide sensor | VIP | VIP1.0 | 360%, 120 nM | https://doi.org/doi:10.1126/science.abq8173 | |
| CCK | CCK1.0 (2.3) | 840%, 4 nM | |||
| NPY | NPY1.0 | 300%, 40 nM | |||
| CRF | CRF1.0 (3.0) | 1200%, 12 nM | |||
| SST | SST1.0 | 430%, 70 nM | |||
| NTS | NTS1.0 | 250%, 6 nM |
Plasmids
Published plasmids are avalible at Addgene, WeKwikGene and MolecularCloud. For the latest unpublished sensors for ACh, DA, 5-HT, NE, eCB, Ado, ATP etc, please contact yulonglilab2018(a)gmail.com for information.
Drosophila
Transgenic flies carrying published ACh sensor and dopamine sensor are available at Bloomington Drosophila Stock Center.
| Genotype | Cat# |
| ACh sensors | |
| w[*]; P{w[+mC]=UAS-GRAB(ACh2.0)}2/In(2LR)Gla, wg[Gla-1] PPO1[Bc] | 80046 |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(ACh3.0)}attP40 | 86549 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(ACh3.0)}VK00005/TM6B, Tb[1] | 86550 |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(ACh3.0)}attP40 | 86551 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(ACh3.0)}VK00005/TM2 | 86552 |
| DA sensors | |
| w[*]; P{w[+mC]=UAS-GRAB(DA1m)}2 | 80047 |
| w[*]; P{w[+mC]=UAS-GRAB(DA1m)}3/TM2 | 80048 |
| y[1] w[*]; P{y[+t7.7] w[+mC]=lexAop-GRAB(DA1m)}attP40/CyO | 80049 |
| y[1] w[*]; PBac{y[+mDint2] w[+mC]=lexAop-GRAB(DA1m)}VK00005 | 80050 |
| w[*]; P{w[+mC]=UAS-DA1m-mut}attP40/CyO | 80051 |
| w[*]; P{w[+mC]=10XUAS-GRAB(DA2m)}attP40/CyO | 90878 |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(DA2m)}attP40/CyO | 90879 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(DA2m)}VK00005/TM2 | 90880 |
| 5-HT sensors | |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(5-HT1.0)}attP40 | 90874 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(5-HT1.0)}VK00005 | 90875 |
| w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(5-HT1.0)}attP40/CyO | 90876 |
| w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(5-HT1.0)}VK00005 | 90877 |
| PARIS | |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP40 | 82174 |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP2 | 82175 |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-pHluorin.CAAX}attP2 | 82176 |
| w[*]; P{y[+t7.7] w[+mC]=10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO | 82177 |
| w[*]; P{y[+t7.7] w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}attP40 | 82178 |
| w[*]; M{w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}ZH-86Fb | 82179 |
Viruses
AAV viruses carrying published and unpublished GRAB sensors (including the lastest ACh, DA, NE, 5-HT, Ado, ATP, VIP, CCK and eCB sensors) are available from Brain Case , BrainVTA (China), WZ Biosciences (China) & WZ Biosciences (US) or Shanghai Taitool Bioscience. Note: please contact the Li Lab (yulonglilab2018@gmail.com) before ordering unpublished sensors.
| Virus | Cat# |
| AAV-hSyn-GACh2.0 | PT-1309 |
| AAV-EF1α-DIO-GACh2.0 | PT-1310 |
| AAV-hSyn-DA1m | PT-1298 |
| AAV-EF1α-DIO-DA1m | PT-1299 |
| AAV-hSyn-DA1h | PT-1301 |
| AAV-EF1α-DIO-DA1h | PT-1302 |
| AAV-hSyn-DA mut | PT-1304 |
| AAV-EF1α-DIO-DA mut | PT-1305 |
| Virus | Cat# |
| pAAV-hSyn-GRAB_DA1m | 113049-AAV9 |
| pAAV-hSyn-GRAB_DA1h | 113050-AAV9 |
| pAAV-hSyn-GRAB_NE1m | 123308-AAV9 |
| pAAV-hSyn-GRAB_NE1h | 123309-AAV9 |
| pAAV-hSyn-GRAB_DA1h | 113050-AAVrg |
| pAAV-hSyn-GRAB_NE1m | 123308-AAVrg |
| pAAV-hSyn-GRAB_NE1h | 123309-AAVrg |
| pAAV-hSyn-GRAB_NEmut | 123310-AAVrg |
For international users, please contact yulonglilab2018(a)gmail.com for information.
