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Updated on October 20, 2024

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GRAB sensors list

Group Ligand Latest Version Peak response and EC50 Receptor backbone Spectrum information
(one-photon)		 Paper link Notes
Green 5-HT sensor 5-HT 5-HT1.0 280%, 22 nM human HTR2C / https://doi.org/10.1038/s41593-021-00823-7 /
5-HTmut / human HTR2C / /
5-HT3.0 1300%, 150 nM mouse HTR4 Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm https://doi.org/10.1038/s41592-024-02188-8 Highly recommend
5-HT3.0mut / mouse HTR4 / /
5-HT2m 1400%, 1.1 uM mouse HTR4 Peak: Ex 505 nm, Em 520 nm Isobestic point: 410 nm /
5-HT2h 600%, 80 nM mouse HTR4 Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm /
Red 5-HT sensor r5-HTmut / mouse HTR4 / /
r5-HT1.0 400%, 790 nM mouse HTR4 Peak: Ex 560 nm, Em 595 nm Highly recommend, previous internal name r5-HT2.0, nondetectable photoactivation
Green ACh sensor ACh gACh2.0 90%, 2 uM human M3R / https://doi.org/10.1038/nbt.4184 /
gACh3.0 (ACh4.3) 280%, 2.2 uM human M3R Peak: Ex 492 nm, Em 510 nm https://doi.org/10.1038/s41592-020-0953-2 Highly recommend
gACh3.0-mut / human M3R / /
Green DA sensor DA DA1m 90%, 130 nM human D2R / https://doi.org/10.1016/j.cell.2018.06.042 /
DA1m-mut / human D2R / /
DA1h 90%, 10 nM human D2R / /
DA1h-mut human D2R / /
DA2m 340%, 90 nM human D2R / https://doi.org/10.1038/s41592-020-00981-9 /
DA2h 280%, 7 nM human D2R / /
gDA3m 1300%, 86 nM human D1R Peak: Ex 495 nm, Em 520 nm Isobestic point: 420 nm https://doi.org/10.1038/s41592-023-02100-w Highly recommend
gDA3h 1000%, 22 nM bovine D1R Peak: Ex 500 nm, Em 520 nm Isobestic point: 410 nm /
Red DA sensor rDA1m 150%, 95 nM human D2R Peak: Ex 565 nm, Em 595 nm https://doi.org/10.1038/s41592-020-00981-9 /
rDA1h 100%, 4 nM human D2R / /
rDA2m(D2) 560%, 180 nM red fire ant D2R Peak: Ex 565 nm, Em 595 nm https://doi.org/10.1038/s41592-023-02100-w Highly recommend, Nondetectable photoactivation
rDA2h(D2) 240%, 9 nM red fire ant D2R Peak: Ex 565 nm, Em 595 nm Nondetectable photoactivation
rDA3m(D1) 1300%, 130 nM human D1R Peak: Ex 560 nm, Em 595 nm Highly recommend
rDA3h(D1) 900%, 20 nM human D1R Peak: Ex 565 nm, Em 585 nm /
Green NE sensor NE NE1m 230%, 930 nM human α2AR / https://doi.org/10.1016/j.neuron.2019.02.037 /
NE1h 130%, 83 nM human α2AR / /
NEmut / human α2AR / Non-binding mutant control
NE2m 381%, 380 nM human α2AR Peak: Ex 500 nm, Em 520 nm Isobestic point: 410 nm https://doi.org/10.1016/j.neuron.2024.03.001 Highly recommend
NE2h 415%, 190 nM human α2AR Peak: Ex 500 nm, Em 520 nm Isobestic point: 410 nm /
Green HA sensor HA HA1h 370%, 17 nM human H4R Peak: Ex 505 nm, Em 520 nm Isobestic point: 400 nm https://doi.org/10.1016/j.neuron.2023.02.024 /
HA1m 590%, 380 nM human H4R Peak: Ex 505 nm, Em 520 nm Isobestic point: 420 nm Highly recommend
HA1mut / human H4R / /
Green OA sensor OA OA1.0 660%, 200nM Drosophila Oct β2R Peak: Ex 500 nm, Em 520 nm https://doi.org/10.1093/nsr/nwae112 /
Green Ado sensor Ado Ado1.0 200%, 60 nM human A2AR / https://doi.org/10.1126/science.abb0556 Highly recommend for physiology
Ado1.0m 400%, 3600 nM human A2AR / https://doi.org/10.1073/pnas.2212387120 Highly recommend for pathology
Green ATP sensor ATP ATP1.0 800%, 80 nM human P2RY1 Peak: Ex 500 nm, Em 520 nm Isobestic point: 435 nm https://doi.org/https://doi.org/10.1016/j.neuron.2021.11.027 /
ATP1.0L 1000%, 32 uM human P2RY1 / /
Green UDP sensor UDP UDP1.0 600%, 90 nM chicken P2Y6 Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm https://doi.org/10.1016/j.neuron.2024.03.017 /
Others VIP VIP1.0 360%, 19 nM human VIPR2 Peak: Ex 500 nm, Em 520 nm Isobestic point: 420 nm https://doi.org/doi:10.1126/science.abq8173 /
CCK CCK1.0 (2.3) 840%, 4.1 nM human CCKBR Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm /
NPY NPY1.0 490%, 43 nM human NPY1R Peak: Ex 500 nm, Em 520 nm Isobestic point: 425 nm /
NPYmut / human NPY1R / /
CRF CRF1.0 (3.0) 1200%, 33 nM human CRFR1 Peak: Ex 500 nm, Em 520 nm Isobestic point: 415 nm /
CRFmut / human CRFR1 / /
SST SST1.0 430%, 70 nM human SSTR5 Peak: Ex 500 nm, Em 520 nm Isobestic point: 425 nm /
NTS NTS1.0 250%, 6.2 nM human NTSR1 Peak: Ex 500 nm, Em 520 nm Isobestic point: 425 nm /
eCB eCB2.0 300%, 0.2uM to AEA, 3uM to 2-AG human CB1R Peak: Ex 500 nm, Em 520 nm https://doi.org/10.1038/s41587-021-01074-4 /
OT(Oxytocin) OT1.0 410%, 3 nM bovine OTR Peak: Ex 505 nm, Em 520 nm Isobestic point: 425 nm https://doi.org/10.1038/s41587-022-01561-2 /
OTmut / bovine OTR / Non-binding mutant control

Plasmids

Published plasmids are avalible at Addgene, WeKwikGene and MolecularCloud. For the latest unpublished sensors for ACh, DA, 5-HT, NE, eCB, Ado, ATP etc, please contact yulonglilab2018(a)gmail.com for information.

Yulong Li Lab
Find and request materials through
Addgene

Drosophila

Transgenic flies carrying published ACh sensor and dopamine sensor are available at Bloomington Drosophila Stock Center.

Genotype Cat#
ACh sensors
w[*]; P{w[+mC]=UAS-GRAB(ACh2.0)}2/In(2LR)Gla, wg[Gla-1] PPO1[Bc] 80046
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(ACh3.0)}attP40 86549
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(ACh3.0)}VK00005/TM6B, Tb[1] 86550
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(ACh3.0)}attP40 86551
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(ACh3.0)}VK00005/TM2 86552
DA sensors
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}2 80047
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}3/TM2 80048
y[1] w[*]; P{y[+t7.7] w[+mC]=lexAop-GRAB(DA1m)}attP40/CyO 80049
y[1] w[*]; PBac{y[+mDint2] w[+mC]=lexAop-GRAB(DA1m)}VK00005 80050
w[*]; P{w[+mC]=UAS-DA1m-mut}attP40/CyO 80051
w[*]; P{w[+mC]=10XUAS-GRAB(DA2m)}attP40/CyO 90878
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(DA2m)}attP40/CyO 90879
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(DA2m)}VK00005/TM2 90880
5-HT sensors
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(5-HT1.0)}attP40 90874
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(5-HT1.0)}VK00005 90875
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(5-HT1.0)}attP40/CyO 90876
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(5-HT1.0)}VK00005 90877
PARIS
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP40 82174
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP2 82175
w[*]; P{y[+t7.7] w[+mC]=10XUAS-pHluorin.CAAX}attP2 82176
w[*]; P{y[+t7.7] w[+mC]=10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO 82177
w[*]; P{y[+t7.7] w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}attP40 82178
w[*]; M{w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}ZH-86Fb 82179

Viruses

AAV viruses carrying published and unpublished GRAB sensors (including the lastest ACh, DA, NE, 5-HT, Ado, ATP, VIP, CCK and eCB sensors) are available from Brain Case , BrainVTA (China), WZ Biosciences (China) & WZ Biosciences (US) or Shanghai Taitool Bioscience. Note: please contact the Li Lab (yulonglilab2018@gmail.com) before ordering unpublished sensors.

BrainVTA (China)

Virus Cat#
AAV-hSyn-GACh2.0 PT-1309
AAV-EF1α-DIO-GACh2.0 PT-1310
AAV-hSyn-DA1m PT-1298
AAV-EF1α-DIO-DA1m PT-1299
AAV-hSyn-DA1h PT-1301
AAV-EF1α-DIO-DA1h PT-1302
AAV-hSyn-DA mut PT-1304
AAV-EF1α-DIO-DA mut PT-1305

Addgene

Virus Cat#
pAAV-hSyn-GRAB_DA1m 113049-AAV9
pAAV-hSyn-GRAB_DA1h 113050-AAV9
pAAV-hSyn-GRAB_NE1m 123308-AAV9
pAAV-hSyn-GRAB_NE1h 123309-AAV9
pAAV-hSyn-GRAB_DA1h 113050-AAVrg
pAAV-hSyn-GRAB_NE1m 123308-AAVrg
pAAV-hSyn-GRAB_NE1h 123309-AAVrg
pAAV-hSyn-GRAB_NEmut 123310-AAVrg

For international users, please contact yulonglilab2018(atAT)gmail.com for information.