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Updated on September 18, 2024
Resources
GRAB sensors list
Group |
Ligand |
Latest Version |
Peak response and EC50 |
Receptor backbone |
Spectrum information (one-photon) |
Paper link |
Notes |
Green 5-HT sensor |
5-HT |
5-HT1.0 |
280%, 22 nM |
human HTR2C |
/ |
https://doi.org/10.1038/s41593-021-00823-7
|
/ |
5-HTmut |
/ |
human HTR2C |
/ |
/ |
5-HT3.0 |
1300%, 150 nM |
mouse HTR4 |
Peak: Ex 505 nm, Em 520 nm
Isobestic point: 425 nm |
https://doi.org/10.1038/s41592-024-02188-8
|
Highly recommend |
5-HT3.0mut |
/ |
mouse HTR4 |
/ |
/ |
5-HT2m |
1400%, 1.1 uM |
mouse HTR4 |
Peak: Ex 505 nm, Em 520 nm
Isobestic point: 410 nm |
/ |
5-HT2h |
600%, 80 nM |
mouse HTR4 |
Peak: Ex 505 nm, Em 520 nm
Isobestic point: 425 nm |
/ |
Red 5-HT sensor |
r5-HTmut |
/ |
mouse HTR4 |
/ |
/ |
r5-HT1.0 |
400%, 790 nM |
mouse HTR4 |
Peak: Ex 560 nm, Em 595 nm |
Highly recommend, previous internal name r5-HT2.0, nondetectable photoactivation |
Green ACh sensor |
ACh |
gACh2.0 |
90%, 2 uM |
human M3R |
/ |
https://doi.org/10.1038/nbt.4184
|
/ |
gACh3.0 (ACh4.3) |
280%, 2.2 uM |
human M3R |
Peak: Ex 492 nm, Em 510 nm |
https://doi.org/10.1038/s41592-020-0953-2
|
Highly recommend |
gACh3.0-mut |
/ |
human M3R |
/ |
/ |
Green DA sensor |
DA |
DA1m |
90%, 130 nM |
human D2R |
/ |
https://doi.org/10.1016/j.cell.2018.06.042
|
/ |
DA1m-mut |
/ |
human D2R |
/ |
/ |
DA1h |
90%, 10 nM |
human D2R |
/ |
/ |
DA1h-mut |
|
human D2R |
/ |
/ |
DA2m |
340%, 90 nM |
human D2R |
/ |
https://doi.org/10.1038/s41592-020-00981-9
|
/ |
DA2h |
280%, 7 nM |
human D2R |
/ |
/ |
gDA3m |
1300%, 86 nM |
human D1R |
Peak: Ex 495 nm, Em 520 nm
Isobestic point: 420 nm |
https://doi.org/10.1038/s41592-023-02100-w
|
Highly recommend |
gDA3h |
1000%, 22 nM |
bovine D1R |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 410 nm |
/ |
Red DA sensor |
rDA1m |
150%, 95 nM |
human D2R |
Peak: Ex 565 nm, Em 595 nm |
https://doi.org/10.1038/s41592-020-00981-9
|
/ |
rDA1h |
100%, 4 nM |
human D2R |
/ |
/ |
rDA2m(D2) |
560%, 180 nM |
red fire ant D2R |
Peak: Ex 565 nm, Em 595 nm |
https://doi.org/10.1038/s41592-023-02100-w
|
Highly recommend, Nondetectable photoactivation |
rDA2h(D2) |
240%, 9 nM |
red fire ant D2R |
Peak: Ex 565 nm, Em 595 nm |
Nondetectable photoactivation |
rDA3m(D1) |
1300%, 130 nM |
human D1R |
Peak: Ex 560 nm, Em 595 nm |
Highly recommend |
rDA3h(D1) |
900%, 20 nM |
human D1R |
Peak: Ex 565 nm, Em 585 nm |
/ |
Green NE sensor |
NE |
NE1m |
230%, 930 nM |
human α2AR |
/ |
https://doi.org/10.1016/j.neuron.2019.02.037
|
/ |
NE1h |
130%, 83 nM |
human α2AR |
/ |
/ |
NEmut |
/ |
human α2AR |
/ |
Non-binding mutant control |
NE2m |
381%, 380 nM |
human α2AR |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 410 nm |
https://doi.org/10.1016/j.neuron.2024.03.001
|
Highly recommend |
NE2h |
415%, 190 nM |
human α2AR |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 410 nm |
/ |
Green HA sensor |
HA |
HA1h |
370%, 17 nM |
human H4R |
Peak: Ex 505 nm, Em 520 nm Isobestic point: 400 nm |
https://doi.org/10.1016/j.neuron.2023.02.024
|
/ |
HA1m |
590%, 380 nM |
human H4R |
Peak: Ex 505 nm, Em 520 nm Isobestic point: 420 nm |
Highly recommend |
HA1mut |
/ |
human H4R |
/ |
/ |
Green OA sensor |
OA |
OA1.0 |
660%, 200nM |
Drosophila Oct β2R |
Peak: Ex 500 nm, Em 520 nm |
https://doi.org/10.1093/nsr/nwae112
|
/ |
Green Ado sensor |
Ado |
Ado1.0 |
200%, 60 nM |
human A2AR |
/ |
https://doi.org/10.1126/science.abb0556
|
Highly recommend for physiology |
Ado1.0m |
400%, 3600 nM |
human A2AR |
/ |
https://doi.org/10.1073/pnas.2212387120
|
Highly recommend for pathology |
Green ATP sensor |
ATP |
ATP1.0 |
800%, 80 nM |
human P2RY1 |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 435 nm |
https://doi.org/https://doi.org/10.1016/j.neuron.2021.11.027
|
/ |
ATP1.0L |
1000%, 32 uM |
human P2RY1 |
/ |
/ |
Green UDP sensor |
UDP |
UDP1.0 |
600%, 90 nM |
chicken P2Y6 |
Peak: Ex 505 nm, Em 520 nm
Isobestic point: 425 nm |
https://doi.org/10.1016/j.neuron.2024.03.017
|
/ |
Others |
VIP |
VIP1.0 |
360%, 19 nM |
human VIPR2 |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 420 nm |
https://doi.org/doi:10.1126/science.abq8173
|
/ |
CCK |
CCK1.0 (2.3) |
840%, 4.1 nM |
human CCKBR |
Peak: Ex 505 nm, Em 520 nm
Isobestic point: 425 nm |
/ |
NPY |
NPY1.0 |
490%, 43 nM |
human NPY1R |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 425 nm |
/ |
NPYmut |
/ |
human NPY1R |
/ |
/ |
CRF |
CRF1.0 (3.0) |
1200%, 33 nM |
human CRFR1 |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 415 nm |
/ |
CRFmut |
/ |
human CRFR1 |
/ |
/ |
SST |
SST1.0 |
430%, 70 nM |
human SSTR5 |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 425 nm |
/ |
NTS |
NTS1.0 |
250%, 6.2 nM |
human NTSR1 |
Peak: Ex 500 nm, Em 520 nm
Isobestic point: 425 nm |
/ |
eCB |
eCB2.0 |
300%, 0.2uM to AEA, 3uM to 2-AG |
human CB1R |
Peak: Ex 500 nm, Em 520 nm |
https://doi.org/10.1038/s41587-021-01074-4
|
/ |
OT(Oxytocin) |
OT1.0 |
410%, 3 nM |
bovine OTR |
Peak: Ex 505 nm, Em 520 nm
Isobestic point: 425 nm |
https://doi.org/10.1038/s41587-022-01561-2
|
/ |
OTmut |
/ |
bovine OTR |
/ |
Non-binding mutant control |
Plasmids
Published plasmids are avalible at Addgene, WeKwikGene and MolecularCloud. For the latest unpublished sensors for ACh, DA, 5-HT, NE, eCB, Ado, ATP etc, please contact yulonglilab2018(a)gmail.com for information.
Drosophila
Transgenic flies carrying published ACh sensor and dopamine sensor are available at Bloomington Drosophila Stock Center.
Genotype |
Cat# |
ACh sensors |
|
w[*]; P{w[+mC]=UAS-GRAB(ACh2.0)}2/In(2LR)Gla, wg[Gla-1] PPO1[Bc] |
80046 |
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(ACh3.0)}attP40 |
86549 |
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(ACh3.0)}VK00005/TM6B, Tb[1] |
86550 |
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(ACh3.0)}attP40 |
86551 |
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(ACh3.0)}VK00005/TM2 |
86552 |
DA sensors |
|
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}2 |
80047 |
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}3/TM2 |
80048 |
y[1] w[*]; P{y[+t7.7] w[+mC]=lexAop-GRAB(DA1m)}attP40/CyO |
80049 |
y[1] w[*]; PBac{y[+mDint2] w[+mC]=lexAop-GRAB(DA1m)}VK00005 |
80050 |
w[*]; P{w[+mC]=UAS-DA1m-mut}attP40/CyO |
80051 |
w[*]; P{w[+mC]=10XUAS-GRAB(DA2m)}attP40/CyO |
90878 |
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(DA2m)}attP40/CyO |
90879 |
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(DA2m)}VK00005/TM2 |
90880 |
5-HT sensors |
|
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(5-HT1.0)}attP40 |
90874 |
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(5-HT1.0)}VK00005 |
90875 |
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(5-HT1.0)}attP40/CyO |
90876 |
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(5-HT1.0)}VK00005 |
90877 |
PARIS |
|
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP40 |
82174 |
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP2 |
82175 |
w[*]; P{y[+t7.7] w[+mC]=10XUAS-pHluorin.CAAX}attP2 |
82176 |
w[*]; P{y[+t7.7] w[+mC]=10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO |
82177 |
w[*]; P{y[+t7.7] w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}attP40 |
82178 |
w[*]; M{w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}ZH-86Fb |
82179 |
Viruses
AAV viruses carrying published and unpublished GRAB sensors (including the lastest ACh, DA, NE, 5-HT, Ado, ATP, VIP, CCK and eCB sensors) are available from Brain Case , BrainVTA (China), WZ Biosciences (China) & WZ Biosciences (US) or Shanghai Taitool Bioscience. Note: please contact the Li Lab (yulonglilab2018@gmail.com) before ordering unpublished sensors.
BrainVTA (China)
Virus |
Cat# |
AAV-hSyn-GACh2.0 |
PT-1309 |
AAV-EF1α-DIO-GACh2.0 |
PT-1310 |
AAV-hSyn-DA1m |
PT-1298 |
AAV-EF1α-DIO-DA1m |
PT-1299 |
AAV-hSyn-DA1h |
PT-1301 |
AAV-EF1α-DIO-DA1h |
PT-1302 |
AAV-hSyn-DA mut |
PT-1304 |
AAV-EF1α-DIO-DA mut |
PT-1305 |
Addgene
For international users, please contact yulonglilab2018(a)gmail.com for information.
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