|
|
Resources
Plasmids
Published plasmids are avalible at Addgene and MolecularCloud. For the latest unpublished sensors for ACh, DA, 5-HT, NE, eCB, Ado, ATP etc, please contact yulonglilab2018(a)gmail.com for information.
Drosophila
Transgenic flies carrying published ACh sensor and dopamine sensor are available at Bloomington Drosophila Stock Center.
Genotype |
Cat# |
ACh sensors |
|
w[*]; P{w[+mC]=UAS-GRAB(ACh2.0)}2/In(2LR)Gla, wg[Gla-1] PPO1[Bc] |
80046 |
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(ACh3.0)}attP40 |
86549 |
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(ACh3.0)}VK00005/TM6B, Tb[1] |
86550 |
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(ACh3.0)}attP40 |
86551 |
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(ACh3.0)}VK00005/TM2 |
86552 |
DA sensors |
|
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}2 |
80047 |
w[*]; P{w[+mC]=UAS-GRAB(DA1m)}3/TM2 |
80048 |
y[1] w[*]; P{y[+t7.7] w[+mC]=lexAop-GRAB(DA1m)}attP40/CyO |
80049 |
y[1] w[*]; PBac{y[+mDint2] w[+mC]=lexAop-GRAB(DA1m)}VK00005 |
80050 |
w[*]; P{w[+mC]=UAS-DA1m-mut}attP40/CyO |
80051 |
w[*]; P{w[+mC]=10XUAS-GRAB(DA2m)}attP40/CyO |
90878 |
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(DA2m)}attP40/CyO |
90879 |
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(DA2m)}VK00005/TM2 |
90880 |
5-HT sensors |
|
w[*]; P{y[+t7.7] w[+mC]=10XUAS-GRAB(5-HT1.0)}attP40 |
90874 |
w[*]; PBac{y[+mDint2] w[+mC]=10XUAS-GRAB(5-HT1.0)}VK00005 |
90875 |
w[*]; P{y[+t7.7] w[+mC]=10XlexAop-GRAB(5-HT1.0)}attP40/CyO |
90876 |
w[*]; PBac{y[+mDint2] w[+mC]=10XlexAop-GRAB(5-HT1.0)}VK00005 |
90877 |
PARIS |
|
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP40 |
82174 |
w[*]; P{y[+t7.7] w[+mC]=QUAS-pHluorin.CAAX}attP2 |
82175 |
w[*]; P{y[+t7.7] w[+mC]=10XUAS-pHluorin.CAAX}attP2 |
82176 |
w[*]; P{y[+t7.7] w[+mC]=10XUAS-ArchT-P2A-mRuby3.CAAX}attP40/CyO |
82177 |
w[*]; P{y[+t7.7] w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}attP40 |
82178 |
w[*]; M{w[+mC]=QUAS-ArchT-P2A-mRuby3.CAAX}ZH-86Fb |
82179 |
Viruses
AAV viruses carrying published and unpublished GRAB sensors (including the lastest ACh, DA, NE, 5-HT, Ado, ATP, VIP, CCK and eCB sensors) are available from Brain Case , BrainVTA (China)or WZ Biosciences (China) & WZ Biosciences (US). Note: please contact the Li Lab (yulonglilab2018@gmail.com) before ordering unpublished sensors.
BrainVTA (China)
Virus |
Cat# |
AAV-hSyn-GACh2.0 |
PT-1309 |
AAV-EF1α-DIO-GACh2.0 |
PT-1310 |
AAV-hSyn-DA1m |
PT-1298 |
AAV-EF1α-DIO-DA1m |
PT-1299 |
AAV-hSyn-DA1h |
PT-1301 |
AAV-EF1α-DIO-DA1h |
PT-1302 |
AAV-hSyn-DA mut |
PT-1304 |
AAV-EF1α-DIO-DA mut |
PT-1305 |
Addgene
For international users, please contact yulonglilab2018(a)gmail.com for information.



|